Rapid communication: SacI restriction fragment length polymorphism in a porcine vascular cellular adhesion molecule (VCAM1) gene2
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منابع مشابه
Rapid communication: SacI restriction fragment length polymorphism in a porcine vascular cellular adhesion molecule (VCAM1) gene.
Probe Source and Description. A randomly selected 1.3-kb cDNA clone (S28) was isolated from a porcine spleen library (CLONTECH j. Amplification and hybridization analysis (previously described; Tuggle and Schmitz, 1994) indicated S28 was specifically expressed in spleen. A 504-bp BgZII-BarnHI subclone of S28 was sequenced and identified as a porcine vascular cellular adhesion molecule ( VCAM) c...
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Polymorphism. A Hinf I PCR-RFLP was identified in the porcine leptin receptor ( LEPR) gene. Source and Description of Primers. Human cDNA (GenBank accession no. U43168) sequence was used to design primers to amplify porcine genomic DNA. Primer Sequences. Forward primer: 5¢GCATCCCATATCTGAACCC3¢; reverse primer: 5¢-CCACTTAAACCATAGCGAATC3¢.
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Source and Description of Probe. A 2.2-kb human cDNA clone for bone morphogenetic protein 5 (BMP5) was excised from the clone U2-16 (Celeste et al., 1990).
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Source and Description of Probe. A 1.1-kb human cDNA clone for bone morphogenetic protein 6 (BMP6) was excised from the clone U2-7 (Celeste et al., 1990). Method of Detection. Genomic DNA was isolated from whole blood and digested with TuqI. Fragments were separated by agarose gel electrophoresis and akaline vacuum-transferred to charged nylon membranes. Hybridizations were at 65°C for 16 to 20...
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PoZymorphism. An RFLP was detected in the superoxide dismutase 2 (SOD2) locus of swine using the restriction enzyme StuI. Source and Description of Probe. An 827-bp human cDNA clone for SOD2 was excised from the EcoRI site of plasmid phMnSOD4 (Xiang et al., 1987). Method of Detection. DNA was isolated from blood, digested with StuI, separated by agarose gel electrophoresis, and transferred ...
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ژورنال
عنوان ژورنال: Journal of Animal Science
سال: 1994
ISSN: 0021-8812,1525-3163
DOI: 10.2527/1994.72102764x